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Characterization and Identification of Substances Isolated from Dialyzer Extracts
Author(s) -
Postaire Eric,
Cohen JeanMichel,
Paul JeanLouis,
Fournier Gilles,
Corvazier Michéle,
Jehenne Guillaume,
Raichvarg Denis,
Man NguyenKhoa
Publication year - 1988
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1988.tb02806.x
Subject(s) - chemistry , ethylene oxide , spectrophotometry , chromatography , gas chromatography , solvent , plasticizer , infrared spectroscopy , nuclear chemistry , organic chemistry , polymer , copolymer
The organic phase of dialyzer dried extracts obtained from Cuprophan hollow fiber and polyacrylonitrile AN 69 parallel‐plate dialyzers, all sterilized by ethylene oxide, were submitted to light and polarized light microscopy, infrared (IR) spectrophotometry, and gas chromatography coupled with mass spectrometry. Colorless polygonal (˜5 times 20 μ.m) and needle‐like (˜3 times 50 μm) crystals were found in great quantity on microscopy examination. IR spectrophotometry of the crystals embedded in potassium bromide disc showed specific spectra in the 1400‐800 cm ‐1 wave number range and typical peaks on wave number assigned for hydroxy or amine (3420 cm ‐1 ), aromatic hydrogen (3060 cm ‐1 ), methyl, methyl‐enic, or methenyl (2960‐2860 cm ‐1 ), and carbonyl (1715 cm ‐1 ) groups can be demonstrated. Seven peaks were detected on gas chromatography of the organic solution containing crystals. Fatty acids and undefined compounds could originate from the dialyzer. Phtalates may leach from blood tubing, used in the rinsing procedure, and BHT is a stabilizer of ethyl ether used as solvent. These compounds could also originate from the dialyzer since phtalates are widely used as a plasticizer and BHT an antioxidant for various polymers.