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Microencapsulated Hepatocytes for Bioartificial Liver Support
Author(s) -
Cai Zhuhui,
Shi Zhiqing,
Geraldine M.,
Sun Anthony M.
Publication year - 1988
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1988.tb02793.x
Subject(s) - bioartificial liver device , collagenase , fulminant hepatic failure , polylysine , perfusion , liver function , liver perfusion , liver failure , chemistry , artificial liver , hepatocyte , medicine , liver transplantation , biochemistry , in vitro , transplantation , enzyme
Free hepatocytes, harvested from normal rat livers by portal vein collagenase perfusion, were encapsulated within alginate‐polylysine membranes and served as a liver support system. The encapsulated hepatocytes remained viable and were able to synthesize protein for up to 3 weeks in culture. Allografts of encapsulated hepatocytes replaced the function of a damaged liver and reduced the mortality rate among rats with galactosamine‐induced fulminant hepatic failure.