Premium
Comparison of Chemical Analyses of Hollow‐Fiber Dialyzer Extracts
Author(s) -
Pearson Frederick C.,
Bohon Joyce,
Lee Wanda,
Bruszer George,
Sagona Mike,
Dawe Robin,
Jakubowski George,
Morrison David,
Dinarello Charles
Publication year - 1984
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1984.tb04293.x
Subject(s) - limulus amebocyte lysate , chromatography , chemistry , cellulose , fiber , membrane , in vitro , biochemistry , medicine , immunology , organic chemistry , lipopolysaccharide
Hollow‐fiber hemodialyzers containing cellulose‐based membranes have been shown to produce positive results with the Limulus amebocyte lysate (LAL) test. This study was undertaken to determine whether endotoxin was causing the reaction. Rinses from 45 parallel‐plate and hollow‐fiber dialyzers from eight different manufacturers were tested using three lysates and four LAL methods. In addition, four in vitro cellular methods‐human leukocytic pyrogen, lymphocytic activating factor, peritoneal macrophage, and arginase release‐were used to evaluate endotoxin activity. The substance causing the reaction was identified using chromatographic methods. Results indicate that the LAL‐reactive material is cellulose derived and is not pyro‐genic.