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Immunoabsorption in an Extracorporeal Plasma Perfusion System: In Vitro Studies
Author(s) -
Burgstaler Edwin A.,
Pineda Alvaro A.
Publication year - 1981
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1981.tb03999.x
Subject(s) - sepharose , chromatography , chemistry , acrylic acid , affinity chromatography , extracorporeal , covalent bond , perfusion , blood proteins , plasma , antibody , extracorporeal circulation , biochemistry , immunology , surgery , medicine , polymer , copolymer , organic chemistry , enzyme , physics , quantum mechanics
A rechargeable plasma perfusion system developed to remove IgG immunoglobulins is described. Partially purified protein A was covalently bonded on two separate carriers, Sepharose 6MB and carboxyl acrylic beads. Plasma from a patient with IgG multiple myeloma was perfused through the Sepharose‐protein. A column, and this resulted in a maximum removal of 177% of the estimated removal capacity of the column. Plasma from the same source was perfused through the acrylic bead‐protein. A column and yielded an average of 220% of the estimated removal capacity of the column. In addition, plasma from a normal subject and from six patients with various autoimmune diseases was perfused through the Sepharose–protein A column, and varying percent removal was obtained. A detailed procedure for covalent coupling of purified protein A to carboxylated acrylic beads is also given.