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Influence of Uremic Middle Molecules on Blood Cells
Author(s) -
Ota Kazuo,
Sanaka Tsutomu,
Agishi Tetsuzo,
Nakajima Osamu
Publication year - 1980
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1980.tb03915.x
Subject(s) - sephadex , chemistry , glutamine , erythrocyte fragility , glycine , red blood cell , uremia , lymphocyte , amino acid , chromatography , biochemistry , medicine , hemolysis , enzyme
Uremic middle molecular weight substances are obtained from the ultrafiltrate of plasma of uremic patients. Seven subfractions are isolated by chromatography using Sephadex G‐15 and DEAE 25 A columns. Each subfraction is tested for its inhibitory effect upon lymphocyte blastogenesis and influence upon osmotic fragility of red blood cells. Consequently, subfractions c and d exert notable effects on both lymphocytes and red blood cells. The main constituents of subfraction d are aspartic acid, glycine, glutamine, and cysteine. It is concluded that uremic middle molecular substances have a deleterious effect upon blood cells. Therefore, hemofiltration, which is advantageous for the removal of middle molecular weight substances, is considered to be a method of choice for uremic patients.