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Enzyme Reactors Consisting of Matrix‐Bound or Entrapped Red Cell Ghosts
Author(s) -
Schmer G.
Publication year - 1980
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1980.tb01866.x
Subject(s) - agarose , membrane , chemistry , enzyme , substrate (aquarium) , urease , in vivo , sephadex , cell , matrix (chemical analysis) , chromatography , biochemistry , in vitro , biophysics , biology , ecology , microbiology and biotechnology
Potent enzyme reactor systems could be synthesized using red cell ghost membranes containing urease and coval‐ently binding these “semi‐artificial” cells to polydextran gels (Sephadex) or entrapping them in agarose gel. In vitro studies as well as in vivo studies in sheep indicate excellent physicochemical stability of the system. Limitations of the method include insufficient diffusion of certain substrates through the cell membrane, or a tight binding of the substrate to carrier proteins.