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Albumin‐Coated Amberlite XAD‐7 Resin for Hemoperfusion in Acute Liver Failure: Part I: Adsorption Studies
Author(s) -
Ton Hsueh Yen,
Hughes Robin D.,
Silk David B. A.,
Williams Roger
Publication year - 1979
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.1979.tb03799.x
Subject(s) - amberlite , human serum albumin , chemistry , adsorption , chromatography , albumin , hemoperfusion , serum albumin , langmuir adsorption model , coating , organic chemistry , biochemistry , surgery , hemodialysis , medicine
Platelets adhere to most foreign surfaces. As a result, polymers and albumin have been suggested as possible coatings to improve the blood compatibility of such surfaces. Amberlite XAD‐7 has a high affinity for human serum albumin (HSA) and several protein‐bound toxic metabolites. In the present study it is shown that HSA can be tightly bound to Amberlite XAD‐7 without. the use of chemical coupling agents. Optimal binding was achieved at pH 5.0 and the adsorption conforms to Langmuir's isotherm. Theoretical analysis of data and absence of a residual surface coating visible with scanning electron microscopy suggest a monolayer of albumin. The amount of HSA eluted from the resin under severe flow conditions was negligible (˜1%). Furthermore, in stirred batch studies with human plasma, the HSA coating did not decrease the adsorptive capacity of XAD‐7 for bile acids and bilirubin.