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Obesity impairs wound closure through a vasculogenic mechanism
Author(s) -
Wagner I. Janelle,
Szpalski Caroline,
Allen Robert J.,
Davidson Edward H.,
Canizares Orlando,
Saadeh Pierre B.,
Warren Stephen M.
Publication year - 2012
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1524-475x.2012.00803.x
Subject(s) - medicine , wound healing , bone marrow , progenitor cell , peripheral , obesity , endocrinology , peripheral blood , endothelial progenitor cell , vasculogenesis , immunology , stem cell , biology , genetics
Since obesity impairs wound healing and bone marrow ( BM )‐derived vasculogenic progenitor cells ( PCs ) are important for tissue repair, we hypothesize that obesity‐impaired wound healing is due, in part, to impaired PC mobilization, trafficking, and function. Peripheral blood was obtained from nondiabetic, obese ( BMI > 30, n = 25), and nonobese ( BMI < 30, n = 17) subjects. Peripheral blood human (h) PCs were isolated, quantified, and functionally assessed. To corroborate the human experiments, 6‐mm stented wounds were created on nondiabetic obese mice (TALLYHO/JngJ, n = 15) and nonobese mice ( SWR / J , n = 15). Peripheral blood mouse (m) PCs were quantified and wounds were analyzed. There was no difference in the number of baseline circulating hPCs in nondiabetic, obese ( hPC ‐ob), and nonobese ( hPC ‐nl) subjects, but hPC ‐ob had impaired adhesion ( p < 0.05), migration ( p < 0.01), and proliferation ( p < 0.001). Nondiabetic obese mice had a significant decrease in the number of circulating PCs ( mPC ‐ob) at 7 ( p = 0.008) and 14 days ( p = 0.003) after wounding. The impaired circulating mPC ‐ob response correlated with significantly impaired wound closure at days 14 ( p < 0.001) and 21 ( p < 0.001) as well as significantly fewer new blood vessels in the wounds ( p < 0.001). Our results suggest that obesity impairs the BM ‐derived vasculogenic PC response to peripheral injury and this, in turn, impairs wound closure.