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Early and late calcium waves during wound healing in corneal endothelial cells
Author(s) -
Chifflet Silvia,
Justet Cristian,
Hernández Julio A.,
Nin Verónica,
Escande Carlos,
Benech Juan C.
Publication year - 2011
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1524-475x.2011.00749.x
Subject(s) - depolarization , calcium , chemistry , biophysics , wound healing , calcium channel , membrane , sodium , biochemistry , medicine , biology , surgery , organic chemistry
Immediately after wounding, bovine corneal endothelial cells develop a fast calcium wave that propagates from the wound border to the rest of the monolayer and extinguishes in approximately 5 minutes. One hour after wounding, a late, slow calcium wave ( SCW ) develops concomitantly to the depolarization of the plasma membrane potential of the border cells. The incorporation of inhibitors of the epithelial sodium channel and of the sodium‐calcium exchanger produces inhibition of the membrane depolarization and the SCW , and diminishes the rate of wound healing. The L‐type calcium channel blocker nimodipine does not have any effect on the SCW . The reversible inhibition of the fast calcium wave does not affect the SCW and only slightly decreases the velocity of healing. Our results suggest that the SCW is at least partially produced by the coupling of the epithelial sodium channel and the sodium‐calcium exchanger functioning in reverse mode. They also suggest that the SCW may play a role in the overall healing process.