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Platelet‐rich fibrin matrix improves wound angiogenesis via inducing endothelial cell proliferation
Author(s) -
Roy Sashwati,
Driggs Jason,
Elgharably Haytham,
Biswas Sabyasachi,
Findley Muna,
Khanna Savita,
Gnyawali Urmila,
Bergdall Valerie K.,
Sen Chandan K.
Publication year - 2011
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1524-475x.2011.00740.x
Subject(s) - angiogenesis , wound healing , fibrin , platelet rich fibrin , extracellular matrix , platelet , vascular endothelial growth factor , growth factor , platelet rich plasma , endothelial stem cell , microbiology and biotechnology , thrombin , chemistry , medicine , immunology , cancer research , biology , biochemistry , receptor , in vitro , vegf receptors
The economic, social, and public health burden of chronic ulcers and other compromised wounds is enormous and rapidly increasing with the aging population. The growth factors derived from platelets play an important role in tissue remodeling including neovascularization. Platelet‐rich plasma ( PRP ) has been utilized and studied for the last four decades. Platelet gel and fibrin sealant, derived from PRP mixed with thrombin and calcium chloride, have been exogenously applied to tissues to promote wound healing, bone growth, hemostasis, and tissue sealing. In this study, we first characterized recovery and viability of as well as growth factor release from platelets in a novel preparation of platelet gel and fibrin matrix, namely platelet‐rich fibrin matrix ( PRFM ). Next, the effect of PRFM application in a delayed model of ischemic wound angiogenesis was investigated. The study, for the first time, shows the kinetics of the viability of platelet‐embedded fibrin matrix. A slow and steady release of growth factors from PRFM was observed. The vascular endothelial growth factor released from PRFM was primarily responsible for endothelial mitogenic response via extracellular signal‐regulated protein kinase activation pathway. Finally, this preparation of PRFM effectively induced endothelial cell proliferation and improved wound angiogenesis in chronic wounds, providing evidence of probable mechanisms of action of PRFM in healing of chronic ulcers.