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Extracellular matrix‐derived tripeptide proline‐glycine‐proline inhibits keratinocyte proliferation and migration
Author(s) -
Ma Yiwei,
Kleinbeck Kyle,
Kao W. John
Publication year - 2011
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1524-475x.2011.00734.x
Subject(s) - proline , tripeptide , glycine , extracellular matrix , microbiology and biotechnology , chemistry , extracellular , keratinocyte , biochemistry , amino acid , in vitro , biology
Keratinocytes are the predominant cell type in epidermis, and are primarily responsible for the epithelialization phase of wound healing. Previous studies by our group showed a positive correlation between IL ‐8 concentration and delayed healing of porcine cutaneous partial‐thickness wounds. Interleukin‐8 and collagen‐breakdown product N ‐acetyl‐ P ro‐ G ly‐ P ro ( PGP ) are known as chemoattractant molecules for neutrophils during inflammation. The activity of both molecules is dependent on chemokine receptors CXCR 1 and CXCR 2. In addition to neutrophils, keratinocytes also express CXCR 1 and CXCR 2. Here we investigated the effects of IL ‐8 and PGP on keratinocyte proliferation and migration. Our results showed that IL ‐8 up to 100 ng/mL does not have any significant impact on keratinocyte proliferation or migration. ECM ‐derived tripeptide PGP chemotactically attracts neutrophils but not keratinocytes. PGP strongly inhibits keratinocyte proliferation and migration in a cell‐type specific manner. Thus, collagen breakdown product PGP plays a key role in modulating both the inflammatory and epithelialization phases of wound healing.