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Selection of proangiogenic ascorbate derivatives and their exploitation in a novel drug‐releasing system for wound healing
Author(s) -
Stumpf Ulla,
Michaelis Martin,
Klassert Denise,
Cinatl Jindrich,
Altrichter Jens,
Windolf Joachim,
Hergenröther Julian,
Scholz Martin
Publication year - 2011
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1524-475x.2011.00718.x
Subject(s) - ascorbic acid , wound healing , angiogenesis , chemistry , fibroblast , dermal fibroblast , pharmacology , biomedical engineering , in vitro , biochemistry , medicine , cancer research , surgery , food science
The pathophysiology leading to delayed wound healing is complex and efficient therapeutic approaches for accelerated wound healing currently do not exist. We developed a novel drug‐eluting platform for the potential use in wound dressings. Here, we report on the potential of eluting ascorbic acid‐2‐phosphate ( ASC ‐2 P ), a highly stable variant of ascorbic acid, to induce angiogenesis and to promote collagen synthesis by fibroblasts. The drug‐eluting platform device ( DEPD ) consists of biocompatible polymeric layers comprising polyethylene terephtalate, polyvinyl alcohol ( PVA ), and polyurethane with PVA as the solvent for ASC ‐2 P . The angiogenic potential of ASC ‐2 P was evaluated in the endothelial cell tube formation assay ( TFA ) and in the chorion allantoic membrane ( CAM ) model. Collagen synthesis by ASC ‐2 P ‐stimulated fibroblasts was determined by S irius R ed staining. ASC ‐2 P significantly induced angiogenesis in five independent TFA and CAM assays and induced collagen synthesis in two different fibroblast cell lines. The eluting kinetics of ASC ‐2 P was determined by the ultraviolet N ano D rop method and the functional 2,2′‐Azinobis‐(3‐ethylbenzthiazolin‐6‐sulfonic acid) method. Eluting profiles showed a continuous release in the range of biologically effective concentrations >10 days. This is the first report showing the proangiogenic‐ and collagen‐promoting features of ASC ‐2 P . DEPD loaded with ASC ‐2 P ought to be further evaluated as wound dressings or as supplementary pads for topical treatment of delayed wound healing in preclinical studies.