Premium
Preconditioning with cobalt protoporphyrin protects human gastric mucosal cells from deoxycholate‐induced apoptosis
Author(s) -
Lawson Tina E.,
Redlak Maria J.,
Yager Dorne R.
Publication year - 2011
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1524-475x.2010.00661.x
Subject(s) - apoptosis , chemistry , dna fragmentation , recombinant dna , microbiology and biotechnology , heme , heme oxygenase , cell culture , inducer , cancer research , biology , biochemistry , programmed cell death , enzyme , gene , genetics
In this study, a known inducer of heme oxygenase‐1 (HO‐1) expression, cobalt protoporphyrin, and the introduction of a recombinant plasmid expressing HO‐1 were examined for their ability to protect gastric epithelial cells from deoxycholate‐induced injury. Physiologic levels of the secondary bile salt induce apoptosis in a human gastric adenocarcinoma mucosal cell line. Cobalt protoporphyrin induced expression of HO‐1 protein with maximal levels attaining a plateau at 48 hours. Pretreatment with cobalt protoporphyrin before challenge with 200 μM deoxycholate inhibited cell death, DNA fragmentation, the appearance of cytosolic nucleosomes, and cleavage of caspase‐3, caspase‐9, and poly‐(ADP‐ribose) polymerase 1. Similarly, expression of HO‐1 by introduction of a recombinant plasmid also showed a resistance to deoxycholate‐induced apoptosis. These results implicate a possible role for HO‐1 in modulating apoptosis in gastric epithelial cells.