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Characterization of endothelial progenitor cells mobilization following cutaneous wounding
Author(s) -
Morris Lee M.,
Klanke Charles A.,
Lang Stephanie A.,
Pokall Stefan,
Maldonado Arturo R.,
Vuletin Jose F.,
Alaee Datis,
Keswani Sundeep G.,
Lim FoongYen,
Crombleholme Timothy M.
Publication year - 2010
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1524-475x.2010.00596.x
Subject(s) - mobilization , progenitor cell , stromal cell , stromal cell derived factor 1 , neovascularization , bone marrow , medicine , wound healing , cxcr4 , endothelial progenitor cell , andrology , stem cell , endocrinology , angiogenesis , immunology , biology , microbiology and biotechnology , inflammation , chemokine , history , archaeology
Bone marrow (BM)‐derived endothelial progenitor cells (EPCs) are known to play an important role in neovascularization and wound healing. We investigated the temporal effects of cutaneous wounding on EPC surface markers within the peripheral blood and BM, and to better understand the role of the stromal cell‐derived factor‐1 alpha (SDF‐1α/CXCR4) axis on EPC mobilization after wounding. FVB/NJ mice were administered bilateral 8 mm circular full‐thickness skin wounds. Peripheral blood and BM were isolated at daily intervals postwounding through day 7 and analyzed for EPC mobilization characteristics and levels of SDF‐1α. Cutaneous wounding was found to cause a transient increase in EPC mobilization that peaked on day 3. In contrast, SDF‐1α protein within blood plasma was observed to significantly decrease on days 3, 4, and 7 following cutaneous wounding. BM levels of SDF‐1α protein decreased to a nadir on day 3, the same day as peak mobilization was observed to occur. The decrease in BM SDF‐1α protein levels was also associated with a decrease in SDF‐1α mRNA suggesting transcriptional down‐regulation as a contributing factor. This study for the first time characterizes EPC mobilization following cutaneous wounding in mice and supports a major role for the SDF‐1α/CXCR4 axis in regulating mobilization within the BM, without evidence for systemic increases in SDF‐1α.