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Use of the parabiotic model in studies of cutaneous wound healing to define the participation of circulating cells
Author(s) -
Song Guodong,
Nguyen Dinh T.,
Pietramaggiori Giorgio,
Scherer Saja,
Chen Bin,
Zhan Qian,
Ogawa Rei,
Yannas I.V.,
Wagers Amy J.,
Orgill Dennis P.,
Murphy George F.
Publication year - 2010
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1524-475x.2010.00595.x
Subject(s) - wound healing , haematopoiesis , bone marrow , context (archaeology) , biology , transgene , green fluorescent protein , regeneration (biology) , immunology , mesenchymal stem cell , peripheral blood , microbiology and biotechnology , stem cell , pathology , medicine , gene , genetics , paleontology
Previous experimental studies to assess the contribution of blood‐borne circulating (BBC) cells to cutaneous wound healing have relied on discontinuous pulsing of labeled BBC elements or bone marrow transplant protocols. Such approaches do not allow the examination of stable BBC cells that have matured in a physiologically normal host. We have used a parabiotic murine model for cutaneous wound healing to evaluate the relative contribution of stable populations of peripheral blood cells expressing the green fluorescent protein (GFP) transgene in otherwise normal animals. Circulating cells (mature and immature) expressing the GFP transgene were easily detected and quantified in wounds of GFP − parabiotic twins during all evaluated stages of the healing response. Using multiple antibody probes, the relative contribution of various subsets of BBC cells could be comparatively assessed. In early wounds, some cells expressing mesenchymal epitopes were documented to be of hematopoietic origin, indicating the utility of this model in assessing cell plasticity in the context of tissue regeneration and repair. Application of this approach enables further investigation into the contribution of peripheral blood in normal and abnormal healing responses.