Premium
Photoacoustic monitoring of granulation tissue grown in a grafted artificial dermis on rat skin
Author(s) -
Hatanaka Kousuke,
Sato Shunichi,
Saitoh Daizoh,
Ashida Hiroshi,
Sakamoto Toshihisa
Publication year - 2010
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1524-475x.2010.00588.x
Subject(s) - biomedical engineering , materials science , dermis , eosin , histology , granulation tissue , laser doppler velocimetry , h&e stain , cd31 , staining , laser , chemistry , pathology , medicine , wound healing , optics , blood flow , immunohistochemistry , surgery , physics
In this study, we investigated the validity of photoacoustic (PA) measurement for monitoring granulation tissue and hence adhesion of grafted artificial dermis (AD). A 2.5 cm × 2.5 cm, 3‐mm‐thick AD composed of an atelocollagen sponge sheet and a silicone film was grafted on a full‐thickness open wound in rat dorsal skin. The grafted AD was irradiated with low‐energy, 532‐nm nanosecond laser pulses to photoacoustically excite blood in neovascularities, and the PA signals induced were measured using a piezoelectric transducer as a function of postgrafting time. The PA signals were compared with results of laser Doppler imaging and histological analysis. We found a significant correlation between the depths of the first or shallowest PA signal peaks and the depths of granulation tissues estimated from histology with hematoxylin & eosin staining ( R =0.951, p <0.05). There was also a significant correlation between the amplitudes of the first PA signal peaks and densities of CD31‐positive cells evaluated from histology with immunohistochemical staining ( R =0.859, p <0.05). With laser Doppler imaging, no clear perfusion signals were observed, which is attributable to a high light scattering loss in ADs. These findings suggest the validity of PA measurement for monitoring the adhesion of grafted ADs.