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A serum amyloid P‐binding hydrogel speeds healing of partial thickness wounds in pigs
Author(s) -
Gomer Richard H.,
Pilling Darrell,
Kauvar Lawrence M.,
Ellsworth Stote,
Ronkainen Sanna D.,
Roife David,
Davis Stephen C.
Publication year - 2009
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1524-475x.2009.00482.x
Subject(s) - fibrocyte , agarose , wound healing , fibroblast , chemistry , myofibroblast , microbiology and biotechnology , pathology , immunology , medicine , biochemistry , biology , in vitro , fibrosis
During wound healing, some circulating monocytes enter the wound, differentiate into fibroblast‐like cells called fibrocytes, and appear to then further differentiate into myofibroblasts, cells that play a key role in collagen deposition, cytokine release, and wound contraction. The differentiation of monocytes into fibrocytes is inhibited by the serum protein serum amyloid P (SAP). Depleting SAP at a wound site thus might speed wound healing. SAP binds to some types of agarose in the presence of Ca 2+ . We found that human SAP binds to an agarose with a K D of 7 × 10 −8 M and a B max of 2.1 μg SAP/mg wet weight agarose. Mixing this agarose 1 : 5 w/v with 30 μg/mL human SAP (the average SAP concentration in normal serum) in a buffer containing 2 mM Ca 2+ reduced the free SAP concentration to ∼0.02 μg/mL, well below the concentration that inhibits fibrocyte differentiation. Compared with a hydrogel dressing and a foam dressing, dressings containing this agarose and Ca 2+ significantly increased the speed of wound healing in partial thickness wounds in pigs. This suggests that agarose/Ca 2+ dressings may be beneficial for wound healing in humans.