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Effects of negative pressure wound therapy on cellular energetics in fibroblasts grown in a provisional wound (fibrin) matrix
Author(s) -
McNulty Amy K.,
Schmidt Marisa,
Feeley Teri,
Villanueva Patricia,
Kieswetter Kris
Publication year - 2009
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1524-475x.2009.00460.x
Subject(s) - wound healing , fibrin , energetics , negative pressure wound therapy , chemistry , extracellular matrix , matrix (chemical analysis) , fibroblast , microbiology and biotechnology , biophysics , medicine , biology , surgery , pathology , biochemistry , immunology , in vitro , chromatography , alternative medicine , ecology
Negative pressure wound therapy (NPWT) using reticulated open cell foam dressing (ROCF) is effective for treatment of recalcitrant wounds; however, the effects of this therapy on cellular metabolism remain to be elucidated. The effect of two different subatmospheric pressure applications on the cell energetics of human fibroblasts grown in a 3D fibrin matrix was studied using two different pressure‐manifolding materials, an ROCF or gauze under suction (GUS). It was found that levels of cytochrome c oxidase, energy charge, and adenosine triphosphate/adenosine diphosphate were significantly increased following the application of NPWT using ROCF vs. GUS ( p <0.05). Increases in these parameters likely reflect an improved energetic status. In addition, levels of transforming growth factor‐β and platelet‐derived growth factor (α and β isoforms) were significantly increased (80 and 53%, respectively; p <0.05) over static control cultures following treatment with NPWT using ROCF but not following GUS. These growth factors are known to be important during wound healing. Clearly, both the material used as the dressing to manifold the subatmospheric pressure and the pressure used have a dramatic effect on cellular response.