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The role of topical negative pressure in wound repair: Expression of biochemical markers in wound fluid during wound healing
Author(s) -
Mouës Chantal M.,
Van Toorenenbergen Albert W.,
Heule Freerk,
Hop Wim C.,
Hovius Steven E. R.
Publication year - 2008
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1524-475x.2008.00395.x
Subject(s) - wound healing , albumin , medicine , matrix metalloproteinase , negative pressure wound therapy , edema , perfusion , tissue inhibitor of metalloproteinase , proteolytic enzymes , pathology , surgery , chemistry , enzyme , biochemistry , alternative medicine
The clinical effects of topical negative pressure therapy (TNP) on wound healing are well described in numerous articles. While the mechanism(s) of action are not completely understood, it is postulated that reduction of local and interstitial tissue edema, increased perfusion of the (peri‐) wound area, changed bacterial composition, and mechanical stimulation of the woundbed contribute to the clinical success. Our hypothesis is that with the removal of excessive fluid, proteolytic enzymes negatively influencing the healing process are removed. Our aim was to assess whether the concentrations of albumin, matrixmetalloproteinase‐9 (MMP‐9), and tissue inhibitor of metalloproteinase (TIMP‐1) were different between wounds treated with TNP and conventional gauze therapy. We analyzed wound fluid samples of 33 wounds treated with either TNP therapy ( n =15) or conventional therapy ( n =18) on albumin, pro‐ and activated MMP‐9, TIMP‐1, and the ratio of total MMP‐9/TIMP‐1. Albumin levels were found to increase significantly in acute wounds compared with chronic wounds; however, no difference could be found on comparing TNP with conventional therapy. We did find significantly lower levels of pro‐MMP‐9 and lower total MMP‐9/TIMP‐1 ratio in TNP‐treated wounds during the follow‐up of 10 days. These data strongly suggest that TNP therapy influences the microenvironment of the wound.