Premium
Tissue profiling MALDI mass spectrometry reveals prominent calcium‐binding proteins in the proteome of regenerative MRL mouse wounds
Author(s) -
Caldwell Robert L.,
Opalenik Susan R.,
Davidson Jeffrey M.,
Caprioli Richard M.,
Nanney Lillian B.
Publication year - 2008
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1524-475x.2007.00351.x
Subject(s) - proteomics , proteome , microbiology and biotechnology , calcium binding protein , regeneration (biology) , phenotype , immunohistochemistry , biology , calmodulin , chemistry , calcium , biochemistry , immunology , gene , organic chemistry
MRL/MpJ‐Fas lpr mice exhibit the ability to regenerate ear tissue excised by dermal punches. This is an exceptional model to identify candidate proteins that may regulate regeneration in typically nonregenerative tissues. Identification of key molecules involved in regeneration can broaden our understanding of the wound‐healing process and generate novel therapeutic approaches. Tissue profiling by matrix‐assisted laser desorption ionization mass spectrometry is a rapid, powerful proteomic tool that allows hundreds of proteins to be detected from specific regions of intact tissue specimens. To identify these candidate molecules, protein expression in ear punches was examined after 4 and 7 days using tissue profiling of MRL/MpJ‐Fas lpr mice and the nonregenerative mouse strain C57BL/6J. Spectral analysis revealed distinct proteomic differences between the regenerative and nonregenerative phenotypes, including the calcium‐binding proteins calgranulin A and B, calgizzarin, and calmodulin. Spatial distributions for these differentially expressed proteins within the injured regions were confirmed by immunohistochemistry.