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Aminated β‐1,3‐ d ‐glucan improves wound healing in diabetic db / db mice
Author(s) -
Berdal Margrete,
Appelbom Hege I.,
Eikrem Jorunn H.,
Lund Åse,
Zykova Svetlana,
Busund LillTove,
Seljelid Rolf,
Jenssen Trond
Publication year - 2007
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1524-475x.2007.00286.x
Subject(s) - wound healing , diabetes mellitus , medicine , placebo , insulin , endocrinology , subcutaneous injection , glucan , chemistry , surgery , biochemistry , pathology , alternative medicine
Delayed wound healing in diabetes is caused by neuropathy, vascular changes, and impaired cellular response to the injury. Macrophages are crucial in normal wound healing, and impaired functions of these cells have been shown in diabetes. β‐1,3‐ d ‐glucans stimulate macrophage function. This open‐label study was performed to see if aminated β‐1,3‐ d ‐glucan (AG) stimulates wound healing in diabetes. Four groups (1–4) of diabetic db/db mice and one nondiabetic control group, db / + (5) were studied: group 1 ( n =11): topical AG; group 2 ( n =10): topical AG and subcutaneous insulin; group 3 ( n =14): topical placebo and subcutaneous insulin; group 4 ( n =10): diabetic control (placebo); group 5 ( n =12): normal control (placebo). At the end of the experiments fasting blood glucose and A1C were (mean ± SE) as follows: Group 1: 30.5 ± 1.9 mmol/L and 11.3 ± 0.6%; group 2: 12.0 ± 1.7 mmol/L and 8.0 ± 0.6%; group 3: 15.4 ± 2.4 mmol/L and 7.4 ± 0.3%; group 4: 32.6 ± 2.6 mmol/L and 12.3 ± 0.6%; group 5: 7.2 ± 0.4 mmol/L and 3.9 ± 0.04%, respectively. The closed wound area was the same in group 1 (AG alone) and group 2 (AG plus insulin) after 17 days, 57.3 ± 4.7 vs. 50.1 ± 4.9% ( p =0.7).The results of these two groups were superior to group 3 (insulin treatment alone, 32.0 ± 4.3%, p <0.001) and diabetic controls (38.2 ± 5.1%, p =0.001). The macrophage‐stimulant AG improves wound healing in db / db mice.