Premium
Collagenase‐2 (MMP‐8) and matrilysin‐2 (MMP‐26) expression in human wounds of different etiologies
Author(s) -
Pirilä Emma,
Korpi Jarkko T,
Korkiamäki Timo,
Jahkola Tiina,
GutierrezFernandez Ana,
LopezOtin Carlos,
SaarialhoKere Ulpu,
Salo Tuula,
Sorsa Timo
Publication year - 2007
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1524-475x.2006.00184.x
Subject(s) - matrix metalloproteinase , matrilysin , collagenase , wound healing , extracellular matrix , basement membrane , stromal cell , keratinocyte , pathology , interstitial collagenase , medicine , chemistry , in vitro , biology , immunology , microbiology and biotechnology , enzyme , biochemistry
Wound healing involves highly controlled events including reepithelialization, neoangiogenesis, and reformation of the stromal compartment. Matrix metalloproteinases (MMPs) are a family of neutral zinc‐dependent endopeptidases known to be essential for the wound‐healing process. MMP‐8 (collagenase‐2) is a neutrophil‐derived highly effective type I collagenase, recently indicated to be important for acute wound healing. MMP‐26 is a more recent and less well‐studied member of the MMP family. Our aim was to study the expression of MMP‐8 and MMP‐26 in human cutaneous wound repair and chronic wounds using histological methods and cell culture. MMP‐8 expression was associated with epithelial cells, neutrophils, and other inflammatory cells in chronic human wounds. MMP‐26 was prominently expressed in the extracellular compartment of most chronic wounds in close vicinity to the basement membrane area. MMP‐26 was also expressed in acute day 1 wounds with declining expression thereafter. In vitro wound experiments showed that both MMP‐8 and MMP‐26 were expressed by migrating human mucosal keratinocytes. Inhibiting MMP‐26 resulted in aberrant keratinocyte migration and proliferation. We conclude that MMP‐8 and MMP‐26 are differentially expressed in acute and chronic wounds.