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Intact Long‐Type dup A as a Marker for Gastroduodenal Diseases in O kinawan Subpopulation, J apan
Author(s) -
Takahashi Ayaka,
Shiota Seiji,
Matsunari Osamu,
Watada Masahide,
Suzuki Rumiko,
Nakachi Saori,
Kinjo Nagisa,
Kinjo Fukunori,
Yamaoka Yoshio
Publication year - 2013
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1111/j.1523-5378.2012.00994.x
Subject(s) - caga , point mutation , frameshift mutation , medicine , helicobacter pylori , odds ratio , cancer , type 2 diabetes , gastroenterology , mutation , gene , genetics , virulence , biology , endocrinology , diabetes mellitus
Background Helicobacter pylori dup A can be divided into two types according to the presence or absence of the mutation. In addition, full‐sequenced data revealed that dup A has two types with different lengths depend on the presence of approximately 600 bp in the putative 5′ region (presence; long‐type and absence; short‐type), which has not been taken into account in previous studies. Methods A total of 319 strains isolated from O kinawa, the south islands of Japan, were included. The status of dup A and cag A was determined by polymerase chain reaction. The presence of mutations in long‐type dup A was determined by DNA sequencing. Results The prevalence of long‐type dup A was 26.3% (84/319). Sequence analysis showed that there were only six cases (7.1%) with point mutations lead to stop codon among 84 long‐type dup A strains studied. Interestingly, intact long‐type dup A without frameshift mutation , but not short‐type dup A , was significantly associated with gastric ulcer and gastric cancer than gastritis ( p = .001 and p = .019, respectively). After adjustment by age, gender, and cag A , the presence of intact long‐type dup A was significantly associated with gastric ulcer and gastric cancer compared with gastritis (odds ratio [ OR ] = 3.35, 95% confidence interval [ CI ] = 1.55–7.24 and OR = 4.14, 95% CI = 1.23–13.94, respectively). Conclusions Intact long‐type dup A is a real virulence marker for severe outcomes in O kinawa, J apan. The previous information gained from PCR ‐based methods without taking long‐type dup A into account must be interpreted with caution.