Premium
IL‐1 Polymorphisms in Children with Peptic Symptoms in South China
Author(s) -
Li Juan,
Wang Fangjin,
Zhou Qiang,
Ou Zhiying,
Jia Hongyun,
Deng Xiaoyan,
He Yunshao,
Wu Xiaoman
Publication year - 2011
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1111/j.1523-5378.2011.00837.x
Subject(s) - helicobacter pylori , gastroenterology , genotype , medicine , gastritis , gastric mucosa , gene , stomach , biology , genetics
Background: Polymorphisms of IL‐1 gene cluster are reported to be associated with histological changes and IL‐1β expression in the gastric mucosa in adults, especially in Helicobacter pylori –infected subjects. As H. pylori infecting adults and children own different virulence genotypes, the aim of this study was to investigate whether IL‐1 polymorphisms are risk factors in young children in South China. Materials and Methods: A total of 128 children with peptic symptoms were enrolled in this study. Polymorphisms of IL‐1B ‐511 and IL‐1B ‐31 were identified by dual fluorescence PCR. Variable number of tandem repeat region in IL‐1RN was detected by conventional PCR and IL‐1β mRNA expression by real‐time PCR ddCT assay. Results: IL‐1B ‐31T and IL‐1B ‐511C were completely linked in this study. Significant differences of IL‐1B ‐511/‐31 genotypes were observed among different clinical outcomes ( p = .001). The IL‐1B‐511TT/‐31CC was mostly found in the moderate gastritis and the above (severe gastritis or gastric ulcer) groups, with percentage of 60.7%. While no association was observed between IL‐1RN genotypes and the gastric mucosal histological changes ( p = .128). Also no relationships were found between IL‐1 polymorphisms and H. pylori infection or gastric mucosal IL‐1β mRNA expression level. Conclusion: Children with IL‐1B ‐511TT/‐31CC may have a risk to develop relatively severe gastric mucosal histological changes in South China.