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Pediatric Helicobacter pylori Infection and Circulating T‐Lymphocyte Activation and Differentiation
Author(s) -
HelminBasa Anna,
Michalkiewicz Jacek,
Gackowska Lidia,
Kubiszewska Izabela,
Eljaszewicz Andrzej,
Mierzwa Grazyna,
Bala Grazyna,
CzerwionkaSzaflarska Mieczyslawa,
Prokurat Andrzej,
Marszalek Andrzej
Publication year - 2011
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1111/j.1523-5378.2010.00809.x
Subject(s) - cd8 , immunology , gastritis , flow cytometry , t cell , helicobacter pylori , t lymphocyte , lymphocyte , inflammation , cd3 , rapid urease test , cytotoxic t cell , biology , immune system , medicine , in vitro , biochemistry
Background: In this study, H. pylori ‐infected and noninfected children with gastritis were compared to a control group with respect to circulating CD4 + and CD8 + T lymphocytes expressing activation and differentiation markers. Additionally, the lymphocyte phenotypes of children with gastritis were correlated with the gastric inflammation scores. Materials and Methods: H. pylori infection status was assessed based on [ 13 C]urea breath test, rapid urease test, and histology. Analysis of the lymphocyte surface molecule expression was carried out by triple‐color flow cytometry. Results: The group of H. pylori ‐infected children showed an elevated proportion of peripheral B cells with CD19 low , along with a twofold increase in the percentage of memory (CD45RO + ) CD4 + and CD8 + T‐cell subsets ( p < .05). Moreover, a positive correlation between the age and the percentage of these subsets was seen ( r = .38, p = .04 and r = .56, p < .01, respectively). Children with gastritis but without infection had a slightly increased percentage of CD8 + T cells and CD56 + NK cells, CD3 high T cells and CD45RO high CD4 + T‐cell subsets ( p < .05). Both H. pylori ‐infected and noninfected children with gastritis were characterized by an increased percentage of memory/effector CD4 + T cells, the presence of NK cells with CD56 high , memory T‐cell subset with CD4 high , and naive, memory, memory/effector, and effector T‐cell subsets with CD8 high ( p < .05). Gastric inflammation scores correlated positively with the percentage of CD4 + T lymphocytes in H. pylori ‐infected children ( r = .42, p = .03). In noninfected children, gastric inflammation scores correlated positively with the percentage of B cells ( r = .45, p = .04). Conclusion: In H. pylori ‐negative children, gastritis was associated with an increased percentage of activated NK and T cells, and intermediate‐differentiated peripheral blood CD4 + T cells, which was more pronounced in H. pylori ‐positive children who also showed an increased B‐cell response. However, increased inflammation was only associated with the elevation of CD4 + T‐cell percentage in H. pylori ‐positive children as well as B‐cell percentage in H. pylori ‐negative children with gastritis.