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Helicobacter pylori cagA Gene Polymorphism Affects the Total Antioxidant Capacity of Human Saliva
Author(s) -
Wnuk Maciej,
Myszka Aleksander,
Lewinska Anna,
Tokarz Iwona,
Solarska Katarzyna,
Bartosz Grzegorz
Publication year - 2010
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1111/j.1523-5378.2009.00731.x
Subject(s) - caga , saliva , urea , helicobacter pylori , chemistry , biochemistry , medicine , gene , virulence
Background: We aimed to evaluate the total antioxidant capacity (TAC) of saliva in healthy Helicobacter pylori ‐positive and negative saliva individuals. Materials and Methods: A total of 102 human saliva samples were checked for the presence of H. pylori DNA ( ureA and cagA gene fragments). TAC of saliva was estimated by ABTS radical cation (ABTS ? + ) decolorization assay. Results: PCR analysis revealed that 36 subjects were ureA‐/cagA‐ , 24 were ureA+/cagA‐ and 42 were ureA+/cagA+ . Smoking habits had no evident effect on H. pylori infection. We found that TAC of the ureA‐/cagA‐ material, after 10 seconds reaction reflecting fast‐reacting antioxidants, was significantly higher than of ureA+/cagA‐ and ureA+/cagA+ samples (p < .01 and p < .001, respectively). Similar results were obtained for reaction time of 3 minutes measuring slow‐reacting antioxidants (p < .001). We also estimated ureA+/cagA‐ and ureA+/cagA+ samples alone and reported a statistically significant decrease in the TAC 3min value of ureA+/cagA+ compared with ureA+/cagA‐ samples (p < .05). Conclusions: Our data demonstrated that altered redox equilibrium may be associated with more frequent occurrence of H. pylori in the saliva samples.