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Eradication of Helicobacter pylori Infection Favourably Affects Altered Gastric Mucosal MMP‐9 Levels
Author(s) -
Kubben Frank J. G. M.,
Sier Cornelis F. M.,
Schram Miranda,
Witte T. Anne M. C.,
Veenendaal Roeland A.,
Van Duijn Wim,
Verheijen Jan H.,
Hanemaaijer Roeland,
Lamers Cornelis B. H. W.,
Verspaget Hein W.
Publication year - 2007
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1111/j.1523-5378.2007.00527.x
Subject(s) - gastroenterology , helicobacter pylori , medicine , gastritis , gastric mucosa , stomach , matrix metalloproteinase , regimen , zymography , antrum , biopsy
Background: Helicobacter pylori gastritis is recognized as an important pathogenetic factor in peptic ulcer disease and gastric carcinogenesis, and is accompanied by strongly enhanced gastric mucosal matrix metalloproteinase‐9 (MMP‐9) levels. Aim: This study was performed to investigate whether H. pylori ‐affected gastric mucosal MMP‐2 and MMP‐9 levels are reversible by successful treatment of the infection. Patients and methods: Fifty‐eight patients with H. pylori ‐associated gastritis were treated with a combination regimen of acid inhibitory therapy and antibiotics for 14 days. The levels and isoforms of MMP‐2 and MMP‐9 were measured by semiquantitative gelatin‐zymography, bioactivity assay and enzyme‐linked immunosorbent assay in gastric mucosal biopsy homogenates. Results: Latent, active, and total MMP‐9 levels decreased consistently and significantly by successful H. pylori eradication, in antrum as well as corpus mucosa, compared with those prior to treatment, irrespective of the therapy regimen used. The elevated levels remained unchanged, however, when treatment failed. MMP‐2 levels did not show major alterations after H. pylori therapy. Conclusion: Elevated MMP‐9 levels in H. pylori‐ infected gastric mucosa are reversible by eradication of the infection. No major changes in mucosal MMP‐2 levels were observed by H. pylori eradication.