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No Correlation of bab A2 with vac A and cag A Genotypes of Helicobacter pylori and Grading of Gastritis from Peptic Ulcer Disease Patients in Brazil
Author(s) -
Mattar Rejane,
Santos Anibal Ferreira dos,
Eisig Jaime Natan,
Rodrigues Tomas Navarro,
Silva Fernando Marcuz,
Lupinacci Renato Micelli,
Iriya Kiyoshi,
Carrilho Flair José
Publication year - 2005
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1111/j.1523-5378.2005.00360.x
Subject(s) - caga , helicobacter pylori , genotype , intestinal metaplasia , gastroenterology , gastritis , rapid urease test , medicine , virulence , bacterial adhesin , gene , biology , genetics
Background. The bab A2 gene, which encodes a blood‐group antigen‐binding adhesin that mediates attachment of Helicobacter pylori to human Lewis b antigens on gastric epithelial cells, has been associated with a higher risk of peptic ulcer and gastric cancer. The purpose of this study was to ascertain the frequency of bab A2 genotype in H. pylori strains of patients with peptic ulcer and to correlate with other virulence factors. Materials and Methods. vac A , cag A, and bab A2 genotypes of H. pylori were determined by using polymerase chain reaction (PCR). DNA was extracted from positive urease test gastric samples of 150 patients with peptic ulcer. Antrum and corpus biopsies were taken for histologic examination according to the updated Sydney system classification. Results. bab A2 genotype was present in 104 (69.3%) and cag A in 113 (75.3%) gastric samples. No significant correlation was observed between bab A2 and vac As1 genotype or between bab A2 and cag A status. The correlation of vac As1 genotype with positive cag A was statistically significant ( p < .001). The bab A2‐positive strain was more frequently found from the gastric samples of men, than of women ( p = .01). Strains harboring cag A, vac As1, and bab A2 genotypes had no association to the grading of gastritis, presence of glandular atrophy, or intestinal metaplasia. The simultaneous presence of cag A, vac As1, and bab A2 was found in 32.6% of the H. pylori strains. Conclusions. bab A2 genotype is frequently found in H. pylori strains from peptic ulcer disease in Brazil, although it has no significant correlation to the worsening of the gastritis and to other virulence markers such as vac As1 and cag A.