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Oral Vaccination Against Helicobacter pylori with Recombinant Cholera Toxin B‐Subunit
Author(s) -
Kubota Eiji,
Joh Takashi,
Tanida Satoshi,
Sasaki Makoto,
Kataoka Hiromi,
Watanabe Katsushi,
Itoh Keisuke,
Oshima Tadayuki,
Ogasawara Naotaka,
Togawa Shouzo,
Wada Tsuneya,
Yamada Tomonori,
Mori Yoshinori,
Fujita Fumitaka,
Shimura Takaya,
Ohara Hirotaka,
Isaka Masanori,
Yasuda Yoko,
Itoh Makoto
Publication year - 2005
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1111/j.1523-5378.2005.00328.x
Subject(s) - helicobacter pylori , gastritis , cholera toxin , microbiology and biotechnology , nasal administration , immunization , antigen , biology , immunology , genetics
Background. The innocuous pure recombinant cholera toxin B‐subunit (rCTB) is very attractive as a strong adjuvant for host immunization, but little is known about rCTB's gastric mucosal immunoadjuvanticity against Helicobacter pylori . The immunoadjuvanticity of rCTB against H. pylori was tested. Material and methods. Mice were immunized with sonicated H . pylori and rCTB orally or intranasally and sacrificed on day 42 after immunization. Passive cutaneous anaphylaxis (PCA) test was performed to evaluate IgE‐mediated anaphylaxis with serum from mice to which H. pylori ‐antigen with rCTB had been administered. Immunoglobulin titer specific to H . pylori in serum, lavation of the gastrointestinal tracts and feces were examined. Gastritis in vaccinated mice after a challenge was assessed with the scoring defined from grading of gastric inflammation. H. pylori proliferation after immunization was investigated by counting colony forming units (CFU) per gram of stomach tissue. Results. PCA test exhibited no reactions against the serum from mice immunized with H . pylori ‐antigen with rCTB administered orally and intranasally. Oral and nasal coadministrations of rCTB significantly raised systemic and mucosal immunities against H. pylori and suppressed proliferation of H . pylori in gastric mucosa. The score of gastritis in mice immunized orally was significantly higher than that of mice immunized nasally due to postimmunization gastritis. Only oral administration of rCTB suppressed H. pylori proliferation as compared with intranasal administration and without rCTB. Conclusions. The present study indicated that rCTB has systemic and mucosal immunoadjuvanticities against H . pylori and that oral vaccination with rCTB might additively support antibiotic eradication.