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Template DNA Ratio can Affect Detection by Genus‐Specific PCR–Denaturing Gradient Gel Electrophoresis of Bacteria Present at Low Abundance in Mixed Populations
Author(s) -
Zhang Li,
Da Stephen,
Grehan Martin,
Lee Adrian,
Mitchell Hazel
Publication year - 2005
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1111/j.1523-5378.2005.00294.x
Subject(s) - temperature gradient gel electrophoresis , biology , polymerase chain reaction , gel electrophoresis , microbiology and biotechnology , helicobacter , bacteria , dna , genus , helicobacter pylori , genetics , 16s ribosomal rna , gene , zoology
The detection of Helicobacter species by genus‐specific polymerase chain reaction–denaturing gradient gel electrophoresis (PCR‐DGGE) was compared with that by species‐specific PCR in murine intestinal samples. Results suggest that, in samples containing multiple Helicobacter species, genus‐specific PCR‐DGGE may fail to detect all Helicobacter species present and that this relates to the initial template DNA ratio.