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Identification of a 23S rRNA Gene Mutation in Clarithromycin‐Resistant Helicobacter pylori
Author(s) -
Stone Gregory G.,
Shortridge Dee,
Flamm Robert K.,
Versalovic James,
Beyer Jill,
Idler Ken,
Zulawinski Laura,
Tanaka S. Ken
Publication year - 1996
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1111/j.1523-5378.1996.tb00043.x
Subject(s) - 23s ribosomal rna , helicobacter pylori , clarithromycin , mutation , identification (biology) , genetics , gene , microbiology and biotechnology , gene mutation , biology , helicobacter , rna , ribosome , botany
Background Transition mutations (A‐G) at residue 2143, cognate to position 2058 in the Escherichia coli 23S rRNA gene, have been shown to confer resistance to macrolides in Helicobacter pylori. This study reports the finding that transversion mutations (A‐C) can occur at 2143 as well. Materials and Methods. Three clarithromycin‐resistant H. pylori isolated from three different patients after treatment with clarithromycin were analyzed for point mutations by cycle sequencing of a 163‐bp amplified region surrounding residue 2143 within the conserved loop of the 23S rRNA gene. Results. Nucelotide sequence comparisons of a 163‐bp amplified product revealed that A‐C transversion mutations occurred at position 2143. H. pylori isolated from the patients prior to treatment were susceptible to clarithromycin and displayed no polymorphism at 2143. Conclusion. This is the first report to show that A‐C transversion mutations at position 2143 can confer resistance to clarithromycin in H. pylori and further support the role that mutations at position 2143 play in conferring macrolide resistance in H. pylori.