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Peptide‐coated vascular grafts: An in vivo study in sheep
Author(s) -
Li Cheng,
Hill Arthur,
Imran Mir,
Tio Fermin
Publication year - 2004
Publication title -
hemodialysis international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.658
H-Index - 47
eISSN - 1542-4758
pISSN - 1492-7535
DOI - 10.1111/j.1492-7535.2004.80412.x
Subject(s) - medicine , jugular vein , in vivo , vascular graft , neointima , anastomosis , external jugular vein , intimal hyperplasia , femoral artery , arteriovenous anastomosis , endothelial stem cell , vein , common carotid artery , carotid arteries , surgery , in vitro , stent , smooth muscle , restenosis , chemistry , biology , biochemistry , microbiology and biotechnology
The data on function and patency of prosthetic vascular grafts in various clinical settings are limited. The purpose of this in vivo study was to compare the function and patency of P15‐coated expanded polytetrafluoroethylene (ePTFE) vascular grafts to uncoated ePTFE grafts in sheep. The P15 cell‐binding peptide was covalently immobilized onto the surface of ePTFE grafts by a novel atmospheric plasma coating method. We evaluated the amount of neointimal tissue ingrowth present at the arterial and venous sides of the anastomoses and the degree of endothelial cell resurfacing of the luminal surface of the graft. Four P15‐coated grafts and two control grafts were implanted as arteriovenous grafts between the femoral artery and vein and the carotid artery and jugular vein in two sheep (n = 6). One animal was euthanized after 14 days and the other after 28 days. The study showed the intimal ingrowth was significantly less. The average intimal thickness of P15‐coated grafts (658 µm) was approximately two and a half times less than that of uncoated samples (1657 µm). The newly formed endothelial cell lining was thicker and its coverage was more uniform for P15‐coated grafts compared to the uncoated controls.