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Unexpected problem in aphid DNA barcoding by universal primers
Author(s) -
LEE Wonhoon,
LEE Seunghwan
Publication year - 2012
Publication title -
entomological science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 28
eISSN - 1479-8298
pISSN - 1343-8786
DOI - 10.1111/j.1479-8298.2011.00473.x
Subject(s) - biology , braconidae , dna barcoding , sitobion avenae , aphid , mitochondrial dna , hymenoptera , dna , parasitoid , zoology , genetics , pest analysis , botany , homoptera , aphididae , gene
DNA barcode (mitochondrial COI ) sequences have allowed for species identification of aphids. In this study, we newly found a DNA barcoding problem in a part of the DNA sequences for Sitobion avenae . Five S. avenae individuals showed differences of, on average, 32.60% in the DNA sequences from other conspecific individuals, and a BLAST search revealed that the five sequences are similar to those of aphid parasitoids such as Aphidius , Ephedrus and Praon spp. (Hymenoptera: Braconidae). Based on these results, we concluded that the universal primers used in aphid DNA barcodes can amplify barcode sequences from parasitoid species within host aphids.