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The emerging roles of placenta‐specific micro RNA s in regulating trophoblast proliferation during the first trimester
Author(s) -
Wang Dan,
Song Weiwei,
Na Quan
Publication year - 2012
Publication title -
australian and new zealand journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.734
H-Index - 65
eISSN - 1479-828X
pISSN - 0004-8666
DOI - 10.1111/j.1479-828x.2012.01481.x
Subject(s) - trophoblast , placenta , microbiology and biotechnology , rna , andrology , pregnancy , biology , medicine , gene , genetics , fetus
In the human placenta, eight ‘placenta‐specific’ micro RNA s (mi RNA s) are exclusively expressed and are associated with high cloning frequencies. The expression of placenta‐specific mi RNA s is known to be involved in preeclampsia, but the understanding of these mi RNA s is still limited. The goal of this study was to investigate the levels and localisations of eight placenta‐specific mi RNA s in placental villi with different proliferative abilities during the first trimester. Immunohistochemical analyses indicated that placental trophoblast proliferation ability was associated with the weight of villi in the same gestational week during the first trimester. Of the eight placenta‐specific mi RNA s, quantitative real‐time RT ‐ PCR demonstrated that the expression of mi R ‐517b and mi R ‐1283 was increased in the lightest villi and decreased in the heaviest one, the expression of mi R ‐519a was increased in the heaviest villi and decreased in the lightest one. In situ hybridisation analysis showed that mi R ‐517b and mi R ‐519a were located primarily in the trophoblast layer, while mi R ‐1283 were expressed not only in the villous trophoblasts but also in some villous stroma cells. These findings suggest that mi R ‐517b and mi R ‐519a may play an important role in trophoblast proliferation during the first trimester.