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Tissue kallikrein activity in pregnancy
Author(s) -
Khedun S M,
Naicker T,
Moodley J
Publication year - 2000
Publication title -
australian and new zealand journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.734
H-Index - 65
eISSN - 1479-828X
pISSN - 0004-8666
DOI - 10.1111/j.1479-828x.2000.tb01180.x
Subject(s) - kallikrein , pregnancy , endocrinology , medicine , preeclampsia , tripeptide , proteinuria , urine , excretion , chemistry , kidney , biology , amino acid , enzyme , biochemistry , genetics
Summary: To determine tissue kallikrein (TK) activity in black African women with hypertensive disorders of pregnancy; 140 women were recruited and divided into the following groups: group A – 35 preeclamptic women, group B – 35 mild to moderate hypertensive pregnant women and group C – 35 normotensive pregnant women, and group D – 35 normotensive non‐pregnant healthy women. The activity of tissue kallikrein was determined from a random untimed urine sample using a selective, synthetic chro‐mogenic tripeptide substrate having the sequence H‐D‐Val‐Leu‐Arg‐pNA (S‐2266). Urinary sodium and potassium levels was determined by flame photometry. Tissue kallikrein activity was decreased in women with preeclampsia (1.54 ± 0.95 vs 3.05 ± 0.83 ngTK/μg protein; p < 0.0001) and mild to moderate hypertensive group (2.03 ± 0.76 vs 3.05 ± 0.83 ngTK/μg protein; p < 0.0001) compared with normotensive pregnant women. There was also a significant difference in tissue kallikrein activity between the pregnancy groups (1.54 ± 0.95 vs 2.03 ± 0.76 ngTK/μg protein; p < 0.001). No difference in tissue kallikrein activity was observed between normotensive pregnant and normotensive non‐pregnant healthy women (3.05 ± 0.83 vs 3.14 ± 0.88 ngTK/μg protein; p = 0.51). There was no difference in the excretion of urinary sodium and potassium in pregnancy groups compared to normotensive pregnant group. Tissue kallikrein activity is decreased in hypertensive disorders of pregnancy.