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A Comparison of Assay Methods for Pregnancy Specific β 1 Glycoprotein (SP‐1)
Author(s) -
Ho P. C.,
Jones W. R.
Publication year - 1980
Publication title -
australian and new zealand journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.734
H-Index - 65
eISSN - 1479-828X
pISSN - 0004-8666
DOI - 10.1111/j.1479-828x.1980.tb00106.x
Subject(s) - radioimmunoassay , radial immunodiffusion , glycoprotein , pregnancy , immunodiffusion , chromatography , chemistry , medicine , microbiology and biotechnology , biology , immunology , antibody , genetics
Summary: Three methods were compared for the assay of pregnancy specific β 1 glycoprotein (SP‐1): radial immunodiffusion (RID), electroimmunodiffusion (EID) and radioimmunoassay (RIA). There was a significant correlation between EID and RIA. A lower, but still significant correlation was found between EID and RID, but wide discrepancies were seen in some cases. This finding suggests heterogeneity of SP‐1 molecule(s). The data indicate that EID is the method of choice for assay of SP‐1 after the tenth week of pregnancy, while the more sensitive RIA should be reserved for use before that stage.