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Histophotometric Determination of Near‐Triploidy in Products of Conception Including Hydatidiform Moles
Author(s) -
Atkin N. B.
Publication year - 1974
Publication title -
australian and new zealand journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.734
H-Index - 65
eISSN - 1479-828X
pISSN - 0004-8666
DOI - 10.1111/j.1479-828x.1974.tb00821.x
Subject(s) - ploidy , mole , partial hydatidiform mole , biology , chromosome , abortion , chromosome number , karyotype , zoology , genetics , pregnancy , fetus , placenta , biochemistry , gene
Summary: Histological sections cut at 50μ, of material from three products of conception showed evidence of triploidy when the DNA contents of the nuclei were measured by a photometric technique in which the cells were flattened by the use of a crushing condenser. A partial mole and a spontaneous abortion showed clear modes in the triploid region. A hydatidiform mole which persisted following evacuation, necessitating cytotoxic therapy, had a wider distribution with evidence for the presence of cells in the diploid, triploid and tetraploid regions. The possible value of this technique in evaluating ploidy levels in products of conception, particularly where chromosome studies are not possible, is discussed.