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The Process of Cervical Regeneration After ElectrocauterizationL:Part 2. Histochemical, Autoradiographic, and pH Study
Author(s) -
Reid Bevan L.,
Singer Albert,
Coppleson Malcolm
Publication year - 1967
Publication title -
australian and new zealand journal of obstetrics and gynaecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.734
H-Index - 65
eISSN - 1479-828X
pISSN - 0004-8666
DOI - 10.1111/j.1479-828x.1967.tb01620.x
Subject(s) - epithelium , basement membrane , regeneration (biology) , thymidine , microbiology and biotechnology , capsule , chemistry , biology , pathology , anatomy , biochemistry , in vitro , medicine , genetics , botany
summary Detailed studies were made of certain aspects of regenerating cells produced in response to destruction of cervical erosions by electrocautery. These aspects were local pH variarions by a polarographic technique, autoradiographic studies, studies of the formationof the epithelial basement membrane and phagocyte properties of the regenerating cells. The range of pH environment over the regenerating area was 7‐7.4. There was evidence indicating that lower values induced a keratinproducing epithelium and higher values induced a mucinproducing epithelium. Labelled thymidine incorporation showed desoxyribose nucleic acid sythesis to be active in cells migrating into the wounded area. It was especially active in presumptive squamous epithelium formed by these cells. Ribosenucleic acid synthesis and amino acid uptake was distributed throughout the regenerating tissue but was especially marked in new squamous epithelium. Evidence is presented that the basement membrane of the epithelium is produced by a restriction of the pericellular glycoprotein capsule of the migrating cells to their basal aspect. The capsule beneath each cell then joins its neighbours laterally. Regenerating cells are actively phagocytic toward spermatozoa.