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The Use of Paper Chromatography in the Recognition of Chemical Damage to Wool
Author(s) -
Köpke Vibeke,
Nilssen Bailli
Publication year - 1957
Publication title -
journal of the society of dyers and colourists
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.297
H-Index - 49
eISSN - 1478-4408
pISSN - 0037-9859
DOI - 10.1111/j.1478-4408.1957.tb02211.x
Subject(s) - cysteic acid , wool , chemistry , hydrolysis , hydrogen peroxide , amino acid analysis , papain , acid hydrolysis , alkali metal , chromatography , methionine , alkaline hydrolysis , amino acid , organic chemistry , cystine , cysteine , biochemistry , materials science , enzyme , composite material
Hydrolysis of wool has been carried out by means of acid, alkali, and papain, the resulting solutions being analysed by one‐ and two‐dimensional chromatography. Acid hydrolysis appears to be the most convenient method. Experiments on desalting the amino‐acid solutions by the method of Consden, Gordon, and Martin show that this cannot usually be employed for purifying the solutions, as cysteic acid, methionine, and probably tyrosinesulphonic acid are destroyed during the process. The method has been used for wool treated with alkali, hydrogen peroxide, and sulphuric acid. In the present form the method is sensitive only to severe damage.