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Tumour necrosis factor‐α promoter region polymorphisms affect the course of spontaneous HBsAg clearance
Author(s) -
Kao PeiChi,
Wu JiaFeng,
Ni YenHsuan,
Lin YingTing,
Chen HueyLing,
HueyJen Hsu Sandy,
Hsu HongYuan,
Chang MeiHwei
Publication year - 2010
Publication title -
liver international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.873
H-Index - 110
eISSN - 1478-3231
pISSN - 1478-3223
DOI - 10.1111/j.1478-3231.2010.02340.x
Subject(s) - hbsag , hbcag , genotype , allele , single nucleotide polymorphism , immunology , hbeag , peripheral blood mononuclear cell , hepatitis b virus , tumor necrosis factor alpha , virology , biology , medicine , gene , virus , genetics , in vitro
Background: This study aimed to investigate the roles of tumour necrosis factor‐α (TNF‐α) gene polymorphisms in the spontaneous clearance of HBsAg after a hepatitis B virus (HBV) infection. Methods: Polymorphisms in the TNF‐α (−1031 T to C, −863 C to A, −857 C to T, −308 G to A and −238 G to A transition) gene were evaluated in 274 chronic HBV‐infected patients and 194 patients with resolved HBV infection. The peripheral blood mononuclear cells (PBMC) isolated from 77 (28%) of the 274 chronic HBV‐infected patients with negative HBeAg and positive antibody to HBeAg were stimulated with HBcAg. Data on TNF‐α genotypes and phenotypes in subjects with/without the A allele at the TNF‐α−863 promoter single nucleotide polymorphism (rs1800630) were compared. Results: The A allele in the −863 promoter region of the TNF‐α gene was present in 154 (56.2%) chronic HBV‐infected patients and 87 (44.8%) patients who recovered from HBV infection (odds ratio 1.58; P <0.01). The TNF‐α−863 A allele genotype predicted lower TNF‐α production by PBMC after in vitro HBcAg stimulation ( P <0.02). Conclusions: The A allele at the −863 locus of the promoter region of the TNF‐α gene predicts lower HBcAg‐inducible TNF‐α secretion. It is also associated with chronicity of HBV infection.