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Hepatitis B viral X protein alters the biological features and expressions of DNA repair enzymes in LO2 cells
Author(s) -
Cheng Bin,
Zheng Yaochu,
Guo Xiaorong,
Wang Ying,
Liu Chunyan
Publication year - 2010
Publication title -
liver international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.873
H-Index - 110
eISSN - 1478-3231
pISSN - 1478-3223
DOI - 10.1111/j.1478-3231.2009.02167.x
Subject(s) - virology , enzyme , dna , biology , chemistry , genetics , biochemistry
Objectives: This study aimed at examining the effects of hepatitis B viral X protein (HBx) on the biological features and the expression of DNA repair enzymes in non‐tumour human hepatic LO2 cells in vitro . Methods: The HBx gene was transfected into LO2 cells to establish stably HBx‐expressing LO2/HBx cells. The morphological features, cell growth, cell cycle, apoptosis and colony formation of LO2/HBx cells, vector‐transfected LO2/pcDNA3.1 cells and unmanipulated LO2 cells were studied. The expressions of DNA repair enzymes and DNA oxidative stress‐related 8‐hydroxydeoxyguanosine (8‐OHdG) were determined by a real‐time quantitative polymerase chain reaction assay and high‐performance liquid chromatography coupled with electrochemical detection respectively. Results: In comparison with controls, significant morphological changes, faster growth, higher frequency of cells at the S phase, but lower at G0/G1 and M/G2 phases, a lower frequency of natural cell apoptosis and a higher percentage of colony formation were observed in the LO2/HBx cells. Furthermore, significantly higher levels of intracellular 8‐OHdG and lower levels of human DNA glycosylase α (hMYHα) mRNA transcripts, but no significant change in human 8‐oxoguanine DNA glycosylase 1 (hOGG1), were detected in the LO2/HBx cells. Conclusions: Our data indicated that HBx promoted growth and malignant transformation of non‐tumour hepatic LO2 cells in vitro , which was associated with the downregulation of hMYHα expression and accumulation of mutagenic DNA adduct 8‐OHdG.