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Genetic differences in oxidative stress and inflammatory responses to diet‐induced obesity do not alter liver fibrosis in mice
Author(s) -
Syn WingKin,
Yang Liu,
Chiang Dian Jung,
Qian Yue,
Jung Youngmi,
Karaca Gamze,
Choi Steve S.,
Witek Rafal P.,
Omenetti Alessia,
Pereira Thiago A.,
Diehl Anna Mae
Publication year - 2009
Publication title -
liver international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.873
H-Index - 110
eISSN - 1478-3231
pISSN - 1478-3223
DOI - 10.1111/j.1478-3231.2009.02036.x
Subject(s) - steatosis , insulin resistance , endocrinology , fibrosis , medicine , nonalcoholic fatty liver disease , cirrhosis , hepatic fibrosis , sirius red , hepatic stellate cell , oxidative stress , biology , obesity , fatty liver , disease
Objective: To determine how genetic factors might influence the progression of nonalcoholic fatty liver disease (NAFLD). Design/Intervention: Beginning in adolescence, male C57BL6 (BL6) and 129/SVJ mice were fed control ( n =15/group) or high‐fat (HF) diets ( n =30/group) for 6 months. Main Outcome Measures: Assessed were body weight, insulin resistance, hepatic production of free radicals, expression of cytokines and fibrosis‐related genes and severity of hepatic steatosis, injury and fibrosis. Results: High‐fat diets induced comparable obesity, hepatic steatosis and insulin resistance in the two strains. Compared with BL6 mice, 129/SVJ mice had impaired induction of antioxidant genes, generated three‐ to four‐fold more free radicals and exhibited two‐fold greater induction of profibrogenic cytokines (interleukin‐4 and transforming growth factor‐β1) and fibrosis‐related genes (fibronectin and tissue inhibitor of metalloproteinase‐1) (all P <0.05 for 129 vs BL6). Surprisingly, however, induction of collagen I α1 mRNA and accumulation of Sirius red‐stained fibrils and hepatic hydroxyproline were similar in BL6 and 129/SVJ mice, and although patchy sinusoidal fibrosis emerged in both strains, neither developed bridging fibrosis. Conclusions: Although BL6 and 129/SVJ mice with diet‐induced obesity, insulin resistance and steatosis differed with respect to several factors that are thought to influence human NAFLD progression, they developed comparable liver fibrosis. Moreover, none of the risk factors for NAFLD‐related cirrhosis in humans, including obesity, insulin resistance, chronic inflammatory and oxidant stress, steatohepatitis or activation of fibrogenic genes, proved to be sufficient to cause cirrhosis in these mice, even when exposure to one or more of these insults was very prolonged.