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Resveratrol inhibits cell growth in a human cholangiocarcinoma cell line
Author(s) -
Roncoroni Leda,
Elli Luca,
Dolfini Ersilia,
Erba Eugenio,
Dogliotti Elena,
Terrani Claudia,
Doneda Luisa,
Grimoldi Maria Grazia,
Bardella Maria Teresa
Publication year - 2008
Publication title -
liver international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.873
H-Index - 110
eISSN - 1478-3231
pISSN - 1478-3223
DOI - 10.1111/j.1478-3231.2008.01749.x
Subject(s) - cell culture , resveratrol , alkaline phosphatase , cell growth , viability assay , lactate dehydrogenase , cell cycle , cell , biology , cancer research , microbiology and biotechnology , chemistry , biochemistry , enzyme , genetics
Background/Aims: Cholangiocarcinoma is a devastating tumour with a poor prognosis. An efficient therapy is unavailable in unoperable patients and new drugs are widely sought for and required. Resveratrol (RES) is a natural molecule with a reported anticancer effect, evaluated on different tumour cell lines. We tested the efficacy of RES on a cholangiocarcinoma cell line for the first time. Methods: We used the human SK‐ChA‐1 cell line, cultured in the classical two‐dimensional model and in the three‐dimensional spheroids. After RES exposure morphology, cell viability (colony‐forming assay), lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and cancer antigen (CA) 19‐9 medium releases, cellular transglutaminase activity, karyotype and cell cycle were evaluated. Results: Resveratrol inhibited cell growth in both the cell culture systems used (from −15 to −80% vs untreated controls) and induced a 40‐fold increase of LDH and ALP activities in the culture medium. Also, transglutaminase (TG) activity increased in the cell lysates, together with a cell cycle perturbation characterised by an accumulation in the G 1 /S phase. Karyotype and CA 19‐9 expression were not influenced by the treatment. Conclusions: The observed cytotoxic effect of RES on the human cholangiocarcinoma SK‐ChA‐1 cell line cultured two‐ and three‐dimensionally suggests to further analyse its chemotherapic/chemopreventive possibilities for this kind of cancer.