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Efficacy of zinc treatment against iron‐induced toxicity in rat hepatoma cell line H4‐II‐E‐C3
Author(s) -
Formigari A.,
Santon A.,
Irato P.
Publication year - 2007
Publication title -
liver international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.873
H-Index - 110
eISSN - 1478-3231
pISSN - 1478-3223
DOI - 10.1111/j.1478-3231.2006.01389.x
Subject(s) - superoxide dismutase , antioxidant , toxicity , metallothionein , glutathione peroxidase , chemistry , zinc , glutathione , metal toxicity , peroxidase , programmed cell death , cell culture , microbiology and biotechnology , biochemistry , pharmacology , nuclear chemistry , enzyme , biology , apoptosis , organic chemistry , genetics
Objective: In this study, we have attempted to explore the possible protection afforded by Zn with regard to its antioxidant potential properties in the iron‐induced toxicity. Methods: Rat hepatoma cell line H4‐II‐E‐C3 was treated with 150 μM ZnSO 4 , 200 μM FeSO 4 or 150 μM ZnSO 4 + 200 μM FeSO 4 for 24 h. We studied the effect of metallothionein (MT), glutathione and metal (Fe and Zn) accumulation. We evaluated the levels of both MT‐1 and MT‐2 mRNAs, activities of antioxidant enzymes, and also determined the distribution of MT and cell death index. Results: The cotreatment produced highest levels of MT. Fe concentration was significantly lower in the Zn–Fe‐treated cells compared with Fe‐treated cells. In both Zn treatments, the superoxide dismutase/glutathione peroxidase ratio was similar to control. The cell death index was lower in the Zn–Fe‐treated cells compared with Fe‐treated cells. Conclusions: Zn may act as antioxidant by competitive inhibition of iron uptake by the divalent metal transporter and/or by MT induction.