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Different growth capacity between infant and adult mouse hepatocytes in vitro correlates to the cyclin D1 level without relation to oxidative DNA damage
Author(s) -
Imamura Emi,
Yamamoto Masahiro,
Miyakoshi Masaaki,
Honmo Satoshi,
Ozaki Atsuko,
Yoshie Masumi,
Tamakawa Susumu,
Yaginuma Yuji,
Kasai Shinichi,
Ogawa Katsuhiro
Publication year - 2005
Publication title -
liver international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.873
H-Index - 110
eISSN - 1478-3231
pISSN - 1478-3223
DOI - 10.1111/j.1478-3231.2005.1125.x
Subject(s) - biology , cyclin d1 , deoxyguanosine , apoptosis , cell cycle , cyclin dependent kinase , hepatocyte , cyclin , cell growth , microbiology and biotechnology , dna synthesis , in vitro , cyclin e , cancer research , endocrinology , oxidative stress , biochemistry
Background: Proliferating capacity of hepatocytes is rapidly decreased during growth into maturity, but its exact reason(s) are not well known. Methods: Hepatocytes isolated from infant (10–14 days old) and adult (10–13 months old) B6C3F1 mice were cultivated in the medium containing epidermal growth factor and insulin. Proliferative capacity, apoptosis, morphological changes, cell cycle proteins and 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG) were compared between the two hepatocyte populations. Results: Although adult hepatocytes rapidly underwent cellular crisis characterized by extended morphology and multiple nuclei without proliferation, infant hepatocytes could proliferate with less crisis. Cyclin D1 was much more abundant in the infant than adult cells, but there was no difference according to the expression of cdk4, cdk2, cyclin E and cdk inhibitors (p16 Ink4 (p16), p21 Cip1/Waf1 (p21) and p27 Kip1 (p27)). 8‐OHdG became high soon after cultivation, while it rapidly went down after day 2 both in the infant and adult cells. Conclusions: The high growth capacity of infant hepatocytes in vitro was dependent on the cyclin D1 level, but there was no relation to 8‐OHdG.