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Enhanced uptake of lactosaminated human albumin by rat hepatocarcinomas: implications for an improved chemotherapy of primary liver tumors
Author(s) -
Di Stefano Giuseppina,
Fiume Luigi,
Bolondi Luigi,
Lanza Marcella,
Pariali Milena,
Chieco Pasquale
Publication year - 2005
Publication title -
liver international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.873
H-Index - 110
eISSN - 1478-3231
pISSN - 1478-3223
DOI - 10.1111/j.1478-3231.2005.1118.x
Subject(s) - hccs , human serum albumin , albumin , asialoglycoprotein receptor , chemotherapy , hepatocyte , chemistry , cancer research , medicine , in vitro , hepatocellular carcinoma , biochemistry
Background/Aims: The hepatocyte receptor for asialoglycoproteins (ASGP‐R) internalizes macromolecules exposing galactosyl residues (MEGRs) which can be used as liver‐addressed drug carriers. This receptor was also found on the cells of the large majority of well differentiated hepatocarcinomas (HCCs). The aim of the present experiments was to ascertain whether ASGP‐R of HCCs is functionally active and these tumors can internalize higher quantities of MEGRs than extra‐hepatic tissues. Methods: We injected radioactive lactosaminated human albumin (L‐HSA) in rats with HCCs produced by nitroso‐diethylamine and measured the radioactivity of tumors, surrounding liver, heart, intestine and kidney. L‐HSA is a MEGR successfully used in humans as a hepatotropic drug carrier. Results: The levels of radioactivity of HCCs were two to three times lower than those of surrounding liver, but several times higher than those of extra‐hepatic tissues. L‐HSA accumulation in the tumors mainly occurred via the ASGP‐R, as indicated by the 20 times lower penetration of non‐lactosaminated HSA. L‐HSA uptake by the well‐differentiated tumors were four times higher compared with that by the poorly differentiated forms. Conclusions: The present results suggest that in the chemotherapy of HCCs expressing the ASGP‐R the extra‐hepatic toxicity of anticancer agents can be reduced by conjugation to L‐HSA.