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Expression of oxidative stress‐related molecules in circulating leukocytes and urine in patients with chronic viral hepatitis
Author(s) -
Saeki Toshiya,
Ichiba Miho,
Tanabe Naotada,
Ueki Masaru,
Okamoto Kinya,
Matsunaga Yoshiko,
Hosho Keiko,
Kanbe Takamasa,
Tsuchiya Hiroyuki,
Kurimasa Akihiro,
Yamada Sadako,
Hirooka Yasuaki,
Hisatome Ichiro,
Kishimoto Yukihiro,
Suou Takeaki,
Murawaki Yoshikazu,
Kawasaki Hironaka,
Yodoi Junji,
Shiota Goshi
Publication year - 2006
Publication title -
liver international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.873
H-Index - 110
eISSN - 1478-3231
pISSN - 1478-3223
DOI - 10.1111/j.1478-3231.2005.01213.x
Subject(s) - oxidative stress , viral hepatitis , catalase , superoxide dismutase , hepatitis , immunology , pathogenesis , medicine
Aims: Oxidative stress plays a role in pathogenesis of chronic viral hepatitis. Expression of oxidative stress‐related molecules remains to be clarified. Methods: 4‐hydroxy‐2‐nonenal (4‐HNE), 4‐hydroxy‐2‐hexenal (4‐HHE), catalase, superoxide dismutase‐1 (SOD‐1), glutathione peroxidase‐1, thioredoxin (TRX) in leukocytes and urinary 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG) were examined in 164 persons, including 130 chronic viral hepatitis patients and 34 normal individuals, by Western blot analysis and enzyme‐linked immunosorbent assay, respectively. Hepatic expression of these proteins was immunohistochemically examined in 12 patients with chronic viral hepatitis, compared with three persons without liver damage. Results: The 4‐HNE/β‐actin ratios in chronic viral hepatitis were significantly higher than those in normal individuals ( P <0.01), and were significantly correlated with asparate aminotransferase (AST) and alanine aminotransferase (ALT) ( P <0.01, each). The catalase/β‐actin and SOD‐1/β‐actin ratios in chronic viral hepatitis were higher than those in normal individuals, and were significantly correlated with 4‐HNE/β‐actin ratios ( P <0.01, each). Hepatic expression of 4‐HNE, 4‐HHE, catalase, SOD‐1 and TRX in chronic viral hepatitis was higher than that without liver damage. Urinary excretion of 8‐OHdG was not changed in chronic viral hepatitis. Conclusions: The results of the present study suggest that expression of oxidative stress‐related molecules in leukocytes is upregulated in relation to serum aminotransferase levels.