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Decreased expression of peroxisome proliferator‐activated receptor α and liver fatty acid binding protein after partial hepatectomy of rats and mice
Author(s) -
Skrtic Stanko,
Carlsson Linda,
Ljungberg Anna,
Lindén Daniel,
Michalik Liliane,
Wahli Walter,
Oscarsson Jan
Publication year - 2005
Publication title -
liver international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.873
H-Index - 110
eISSN - 1478-3231
pISSN - 1478-3223
DOI - 10.1111/j.1478-3231.2004.0998.x
Subject(s) - peroxisome proliferator activated receptor alpha , peroxisome proliferator activated receptor , peroxisome , fatty acid binding protein , hepatectomy , chemistry , receptor , fatty liver , medicine , endocrinology , nuclear receptor , biology , biochemistry , gene , transcription factor , surgery , disease , resection
Background Aims: Marked changes in metabolism, including liver steatosis and hypoglycemia, occur after partial hepatectomy. Peroxisome proliferator‐activated receptor α (PPARα) is a nuclear hormone receptor that is activated by fatty acids and involved in hepatic fatty acid metabolism and regeneration. Liver fatty acid binding protein (LFABP) is an abundant protein in liver cytosol whose expression is regulated by PPARα. It is involved in fatty acid uptake and diffusion and in PPARα signaling. The aim of this study was to investigate the expression of PPARα and LFABP during liver regeneration. Methods: Male Sprague–Dawley rats and male C57 Bl/6 mice were subjected to 2/3 hepatectomy and LFABP and PPARα mRNA and protein levels were measured at different time points after surgery. The effect of partial hepatectomy was followed during 48 h in rats and 72 h in mice. Results: PPARα mRNA and protein levels were decreased 26 h after hepatectomy of rats. The LFABP mRNA and protein levels paralleled those of PPARα and were also decreased 26 h after hepatectomy. In mice, the mRNA level was decreased after 36 and 72 h after hepatectomy. In this case, LFABP mRNA levels decreased more slowly after partial hepatectomy than in rats. Conclusions: A marked decrease in PPARα expression may be important for changed gene expression, e.g. LFABP, and metabolic changes, such as hypoglycemia, during liver regeneration.