Premium
Ultrasound detection of spontaneous hepato‐cellular carcinomas in X/ myc bitransgenic mice
Author(s) -
Mai W.,
Barraud L.,
Lefrancois L.,
Scoazec J.Y.,
Guerret S.,
Vray D.,
Merle P.,
VitvitskiTrepo L.,
Trepo C.,
Janier M.F.
Publication year - 2004
Publication title -
liver international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.873
H-Index - 110
eISSN - 1478-3231
pISSN - 1478-3223
DOI - 10.1111/j.1478-3231.2004.0955.x
Subject(s) - ultrasound , medicine , histopathology , hepatocellular carcinoma , radiology , pathology
Aims: To evaluate trans‐abdominal ultrasound for the detection of Hepatocellular carcinoma (HCC) in a bitrasgenic murine ( X / myc ) model using a commercially available high‐frequency ultrasound unit. Methods: Sixty‐one female animals were included in this study. These animals were submitted to a single ultrasound examination of the liver under general anesthesia (isoflurane), and then euthanized. Results of ultrasound were compared with necropsy and histopathology. Results: The lesions demonstrated a fairly consistent aspect (oval‐ or round‐shaped, well‐defined hypoechoic homogeneous lesions), and lesions as small as 2 mm were identified. For detection of hepatic nodules per mouse the sensitivity was 75%, the specificity was 100% and the accuracy was 88.5%. For detection of hepatic focal lesions per lesions the overall sensitivity was 60%, the specificity was 97%, and the accuracy was 75.9%. Contrast‐enhanced harmonic ultrasound imaging did not improve the identification of the lesions in our experimental conditions. Conclusion: High‐frequency ultrasound appears to be an efficient tool allowing new possibilities to use this animal model and evaluate new therapies in longitudinal studies, which are much more powerful.