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Characteristics of murine histidinaemia and its potential for genetic manipulation
Author(s) -
Mellor N.,
Themis M.,
Selden C.,
Jones M.,
Hodgson H. J. F.
Publication year - 2004
Publication title -
liver international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.873
H-Index - 110
eISSN - 1478-3231
pISSN - 1478-3223
DOI - 10.1111/j.1478-3231.2004.0929.x
Subject(s) - in vivo , catabolism , histidine , transduction (biophysics) , gene , biology , complementary dna , in vitro , enzyme , biochemistry , gene expression , cytosol , microbiology and biotechnology , genetics
Background: Histidinaemia is an autosomal recessive disorder affecting the hepatic enzyme histidine ammonia lyase (histidase) resulting in elevated plasma and urinary histidine and is prototypic of a series of hepatic cytosolic enzyme defects. Aims: To characterise the physiology of murine histidinaemia with respect to histidine excretion and catabolism, and explore the potential for manipulating cellular and whole body histidase metabolism by gene transfer. Materials and Methods: We studied his/his mice which have a G to A substitution in the gene encoding histidase, using both in vitro transduction of isolated hepatocytes by lipofection with wild‐type histidase cDNA, and in vivo transduction of whole liver using a retroviral construct. Results and Conclusion: Histidase cDNA expression restored histidase activity in vivo and in vitro towards normal levels, demonstrated both at the cellular level and by whole body metabolic studies, establishing the potential of this model for the development of new gene therapeutic approaches.